Ferdowsi University of Mashhad
Department of Medical Sciences
Ferdowsi University of Mashhad
Ferdowsi University of Mashhad
Plasmodium was frst identifed in a goat in Angola in 1923, and only recently characterized by DNA isolation from a goat blood sample in Zambia. Goats were frst domesticated in the Fertile Crescent approximately 10,000 years ago, and are now globally distributed. It is not known if the Plasmodium identifed in African goats originated from parasites circulating in the local ungulates, or if it co-evolved in the goat before its domestication. To address this question, we performed PCR-based surveillance using a total of 1,299 goat blood samples collected from Sudan and Kenya in Africa, Iran in west Asia, and Myanmar and Thailand in southeast Asia. Plasmodium DNA was detected from all locations, suggesting that the parasite is not limited to Africa, but widely distributed. Whole mitochondrial DNA sequences revealed that there was only one nucleotide substitution between Zambian/Kenyan samples and others, supporting the existence of a goat-specifc Plasmodium species, presumably Plasmodium caprae, rather than infection of goats by local ungulate malaria parasites. We also present the frst photographic images of P. caprae, from one Kenyan goat sample.
Aim: Schmallenberg virus (SBV) is a newly emerging virus in Simbu group that 1st time is reported in 2011 in Germany and now spread to Europe. The clinical signs of infection to this virus are fever, loss of appetite, reduced milk yield and in some cases, diarrhea and in pregnant animals congenital malformations in calves, lambs, and kid goats. Materials and Methods: In this study for a serologic survey of SBV, blood samples from 200 horse in different rural areas of the northern and northeast of Iran with the high equine population collected and were analyzed using an indirect ELISA test. Results: Based on our results 5% (n=10) of total 200 samples were positive for SBV antibody and 2% (n=4) was doubtful and 93% (n=186) was negative. There were no significant differences between age and sex and breed properties (p>0.05). Conclusion: This study demonstrated the presence of antibodies against the SBV on horse populations in Iran. The high population and activity of Culicoides biting midges and their proper living conditions, especially the areas of temperate and humid environmental conditions, are the possible causes of arboviruses related diseases seen in this country.
Cystic hydatidosis is an important zoonosis, which is significant in terms of economics and public health. This disease is endemic in Iran and different livestock act as intermediate host. This disease is a serious public health problem and cause economic losses due to abattoir condemnation of infected organs. The aim of this study is to determine the prevalence and fertility rate of liver and lung hydatid cysts of sheep and cattle in northeast of Iran. A total of 3700 sheep and 1673 cattle were inspected and infected livers and lungs were collected. The overall prevalence rate was 1.4% and 6.75% in sheep and cattle, respectively. Hydatid cyst in lung is more than liver cyst in cattle. In sheep, hydatid cysts were recovered from 0.81% of the liver and 0.59% of the lung. Statistical analysis revealed the age-dependent prevalence of hydatid cysts in cattle and infection rate increase with age. Gender is an important factor in both cattle and sheep. The prevalence rate was 5.2% in ewes and 1.13% in rams. In contrast, hydatid cysts were more common in male than female cattle. The highest and lowest rate of fertility were observed in pulmonary cysts of sheep and pulmonary cysts in cattle, respectively. High fertility rate of sheep cysts show the great potential risk of sheep in the mentioned area
Objective: To detect the presence or absence of EHV-1 and EHV-4 in North-East equine population of Iran. Material and methods: Blood samples of 200 adult horses located in 80 different rural areas of North-East of Iran, were examined for Equine herpesvirus-1 and 4 presences. Absolute quantitation of EHV-4 target molecules was performed using standard curves and the detection limit of the assay was shown to be six copies per reaction. Results: Our study showed a high prevalence of EHV-4 (88%) in these regions. EHV-1 DNA was not detected in any sample. Conclusion: In addition to previous serological study, our report is the frst to detect the EHVs in blood samples of Iran’s equine population by using a high sensitive real-time PCR diagnostic assay and it provides new information for the virus distribution map
The equine herpesviruses (EHVs) are important pathogens in all members of Equidae family world wide. Because of the causative agents of several diseases and latency makes major challenges to equine industry. In the present study blood samples of 108 mules and donkeys from different rural areas of North-East of Iran, as a region with most equine population, were examined for presence of Equine herpesvirus -1 and 4 using real time PCR as a rapid molecular diagnostic test that has high sensitivity and specificity. Based on results, it was conclude that EHV-1 DNA was not detected in any blood samples whereas EHV-4 was present in 14.8% of donkeys and mules. Which can have important role as a source of infection for other equids population.
The breeding of camels in the world is growing expansionary due to increasing in consumption rate of its meat, milk and wool. Besides, The rise of profitability of this industry and importing of camels into the country through the South East borders make it important to recognize infectious diseases in this species. Theileriosis a fatal protozoan infection with worldwide distribution into the domestic animals. It has two important species, Theileria camelensis, and Theileria Dromedarii. Because of low reliability in the current visual detection of this diseases we designed this study to investigate the prevalence of camels Theileria in Zabol and Zahedan district. Blood of 310 tick infected camels were collected from the jugular vein during July 2014 to July 2005. DNA was extracted from a whole blood sample, and PCR determined the presence of the parasite.The results showed that none of the blood contains the DNA of Theileri Camelensis and Theileria Dromedarii This result suggests that this population of camels in the southeast of country is clean from the Theileri Camelensis and Theileria Dromedarii.